Biofilms2Graph

Deep learning–based high-information-content graph representation of early stage bacterial biofilms

From microscopy images to interaction graphs

An AI-driven computational framework for high-resolution analysis of early-stage bacterial biofilms. The system integrates deep learning, microscopy, and graph-based modeling to uncover non-obvious patterns of microbial organization.

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Key capabilities

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Deep learning pipeline

Automated cell segmentation using Mask R-CNN combined with a custom neural network (BINet) for intercellular interaction prediction.

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Graph-based modeling

Representation of biofilms as undirected interaction graphs, where cells are vertices and predicted interactions are edges.

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Quantitative analysis

Extraction of graph features for studying biofilm growth, identifying recurrent structural motifs, and classifying colonization patterns.

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Predictive insights

Prediction of developmental stage and substrate material type directly from image-derived graph representations.

About the project

Bacterial biofilms are spatially organized microbial communities that exhibit increased resistance to antibiotics and play a key role in chronic infections. Understanding their structure at early formation stages is essential for developing effective intervention strategies.

Biofilms2Graph provides a scalable, high-information-content framework for automated biofilm analysis. By combining microscopy visualization with deep learning and graph theory, the platform enables systems-level exploration of microbial community organization and reveals previously hidden structural patterns.

📖 How to cite

Authors: Lev E. Nersesyan, Daniil A. Boiko, Saniyat Kurbanalieva, Lilya U. Dzhemileva, Konstantin S. Kozlov, Valentine P. Ananikov

Title: Deep learning-based high-information-content graph representation of early stage bacterial biofilms

Year: 2026 | Version: 1.0

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